Bologna ensured Napoli would finish the weekend 18 points clear at the top of Serie A thanks to a 1-0 win over Inter Milan on Sunday.
Riccardo Orsolini scored the only goal of the game in the 76th minute to move Bologna up to seventh and leaders Napoli closer to their first league title since 1990.
Thiago Motta’s side also had Musa Barrow’s goal disallowed for offside and hit the bar past Roberto Soriano in the first half.
They are six points behind Atalanta who are at AC Milan in their last game on Sunday and sit in the last European place in sixth.
Bologna also moved above Juventus who were hoping for a fourth straight league win against local rivals Torino on Tuesday after crushing Nantes to reach the last 16 of the Europa League in midweek.
Second-placed Inter have a chance to move back to within 15 points of Napoli, who beat Empoli 2-0 on Saturday, with a win.
But Simone Inzaghi’s side looked weakened after Wednesday’s Champions League first-leg win over Porto and could now be caught by Milan and Roma.
Milan are three points behind Inter as Roma – who are level on 44 points with the reigning champions – travel to bottom side Cremonese on Tuesday.